Isolation, identification and characterization of Lactobacillus plantarum from camel milk and its antagonist effect against diarrheal bacteria.

  • Meriem Toualbia, Abd ElKader Delmi Bouras Laboratory of Natural Local Bio-Resources, Department of Biology, Faculty of Science, Hassiba Benbouali University, Chlef, BP. 151 Hay Essalem, Chlef 02000, Algeria.
  • Malika Koiche, Mohamed Kerkoud DiagGene, 8, Rue le Notre 49066 Angers, France

Abstract

The main objective of this study is to define probiotic bacteria efficiency against bacterial infantile diarrhea. Lactic acid bacteria (LAB) was used as a natural resource and extracted of camel milk from southern Algeria. The isolation was followed by molecular identification using the 16S rDNA sequencing method. One hundred and fifty fecal samples were collected over 18 months from children suffering from diarrhea and aged 2 years or under. Identification results reveal the isolated lactic bacteria as Lactobacillus plantarum. In the other side, a total of 120 fecal samples were positive for bacterial growth, these pathogenic bacteria were identified as: Escherichia fergusonii (92%), Salmonella enterica subsp. Diarizonae (7.33%) and Proteus mirabilis (0.66%). The inhibitory effect study of lactic acid bacteria on pathogenic bacteria shows varying effects of L. plantarum in relation to the various pathogenic isolates, mentioning that the most important effect was expressed against Escherichia fergusonii with 22 mm. All the results allow us to classify that camel's milk is a natural source rich in lactic acid bacteria, in particular L. plantarum, with an inhibitory ability against the pathogenic bacteria responsible for diarrhea. Therefore L. plantarum produces bioactive molecules responsible for this effect.

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How to Cite
Delmi Bouras, M. T. A. E., and M. K. Mohamed Kerkoud. “Isolation, Identification and Characterization of Lactobacillus Plantarum from Camel Milk and Its Antagonist Effect Against Diarrheal Bacteria.”. Emirates Journal of Food and Agriculture, Vol. 30, no. 4, May 2018, pp. 283-7, doi:https://doi.org/10.9755/ejfa.2018.v30.i4.1663. Accessed 18 Dec. 2018.