Determination of pesticides residues in quinoa (Chenopodium quinoa Willd) using QuEChERS and LC-MS/MS

  • Franz Zirena Vilca Escuela de Posgrado de la Universidad Nacional del Altiplano, Av Floral 1153 Puno-Perú
  • Graziela Cristina Rossi Moura Andrade Centro de Energia Nuclear na Agricultura, Laboratorio de Ecotoxicologia, Avenida Centenario 303, 13416-903 Piracicaba, SP, Brazil
  • Walter Alejandro. Zamalloa Cuba Escuela Profesional de Ingeniería Quimica de la Universidad Nacional del Altiplano, Av Floral S/N Puno-Peru
  • Valdemar Luiz. Tornisielo Centro de Energia Nuclear na Agricultura, Laboratorio de Ecotoxicologia, Avenida Centenario 303, 13416-903 Piracicaba, SP, Brazil

Abstract

The QuEChERS method was optimized and validated for the determination of 40 pesticides in 37 samples of quinoa from the harvest 2010-2011 in Puno - Peru. The method involves extraction with 1% acetic acid acetonitrile with CH3COONa anhydrous and MgSO4 followed by dispersive solid-phase extraction with PSA and C18. Analyses were performed with LC-MS/MS using C18 column 150 mm x 2.1 x 3.5 µm, water and acetonitrile 0.1% formic acid used as mobile phase. Spiked quinoa blank at 0.01 and 0.1 mg kg-1 was employed to evaluate recovery ranging from the 31.6 and 125.1% with RSD ≤ 23. The method showed linearity r2 ≥ 0.99 and limits of quantification ranged from 0.001 and 0.01 mg kg-1, and was not observed a matrix effects.  No pesticides residues were observed in the analyzed samples.

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How to Cite
Vilca, F. Z., G. C. R. Moura Andrade, W. A. Zamalloa Cuba, and V. L. Tornisielo. “Determination of Pesticides Residues in Quinoa (Chenopodium Quinoa Willd) Using QuEChERS and LC-MS/MS”. Emirates Journal of Food and Agriculture, Vol. 30, no. 5, May 2018, pp. 421-7, doi:https://doi.org/10.9755/ejfa.2018.v30.i5.1683. Accessed 4 July 2020.
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Short Communication