Effect of ultrasound pretreatment on the antioxidant capacity and antihypertensive activity of bioactive peptides obtained from the protein hydrolysates of Erythrina edulis

  • Carlos Martín Guerra-Almonacid Departamento de Química, Grupo de Investigación de Productos Naturales, Facultad de Ciencias. Universidad del Tolima. Ibagué, Colombia
  • Juan Gabriel Torruco-Uco Tecnológico Nacional de México/Instituto Tecnológico de Tuxtepec, Av. Dr. Victor Bravo Ahuja S/N., Col. 5 de Mayo, Tuxtepec, Oaxaca, C.P. 68350, México
  • Walter Murillo-Arango, Jonh Jairo Méndez-Arteaga Departamento de Química, Grupo de Investigación de Productos Naturales, Facultad de Ciencias. Universidad del Tolima. Ibagué, Colombia
  • Jesús Rodríguez-Miranda Tecnológico Nacional de México/Instituto Tecnológico de Tuxtepec, Av. Dr. Victor Bravo Ahuja S/N., Col. 5 de Mayo, Tuxtepec, Oaxaca, C.P. 68350, México

Abstract

The aim of this research was to evaluate the effect of ultrasound pretreatment on enzymatic hydrolysis with Flavourzyme® and Alcalase® enzymes and the ACE-inhibitory activity and the antioxidant capacity of protein hydrolysates of Erythrina edulis. A protein concentration of 78.8% was obtained after sonicating the protein solutions (10%, w/v) for 10 min at 80 kHz and 100% amplitude. The ultrasonic pretreatment (UP) increased the degree of hydrolysis (47.7%) up to 70 min, the IC50 values in both samples [with (UP) and without pretreatment (WP)] were 100 μg/mL, and the UP samples presented the highest percentage of inhibition at 57.3%. The UP hydrolysates showed the highest (p < 0.05) antioxidant (ABTS*) and radical (DPPH*) - scavenging activities, with IC50 values ranging from 64.52 to 77.62 μg/mL and from 151.13 to 173.22 μg/mL, respectively. In SDS-PAGE, the hydrolysates UP exhibited low molecular weight bands (8 - 20 kDa). The results of both, antioxidants and antihypertensive activities obtained in vitro, showed a higher percentage of activity for the peptides obtained after pretreatment with ultrasound than for those obtained without the use of ultrasound prior to enzymatic hydrolysis.

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