IDENTIFICATION OF β-NERVE GROWTH FACTOR IN DROMEDARY CAMEL SEMINAL PLASMA AND ITS ROLE IN INDUCTION OF OVULATION IN FEMALES

  • Meriem Fatnassi Livestock and Wildlife Laboratory, Arid Lands Institute, 4100 Médenine, University of Gabès, Tunisia
  • José Alvaro Cebrián-Pérez Department of Biochemistry and Molecular and Cell Biology, IUCA, School of Veterinary Medicine, University of Zaragoza-IUCA, Zaragoza, Spain
  • Imed Salhi Livestock and Wildlife Laboratory, Arid Lands Institute, 4100 Médenine, University of Gabès, Tunisia
  • Rosaura Pérez-Pé Department of Biochemistry and Molecular and Cell Biology, IUCA, School of Veterinary Medicine, University of Zaragoza-IUCA, Zaragoza, Spain
  • Mabrouk Mouldi Seddik Livestock and Wildlife Laboratory, Arid Lands Institute, 4100 Médenine, University of Gabès, Tunisia
  • Adriana Casao Department of Biochemistry and Molecular and Cell Biology, IUCA, School of Veterinary Medicine, University of Zaragoza-IUCA, Zaragoza, Spain
  • Touhami Khorchani Livestock and Wildlife Laboratory, Arid Lands Institute, 4100 Médenine, University of Gabès, Tunisia
  • Teresa Muiño-Blanco Department of Biochemistry and Molecular and Cell Biology, IUCA, School of Veterinary Medicine, University of Zaragoza-IUCA, Zaragoza, Spain
  • Mohamed Hammadi Livestock and Wildlife Laboratory, Arid Lands Institute, 4100 Médenine, University of Gabès, Tunisia

Abstract

The main objective of this study was to demonstrate the effect of seminal plasma ovulation inducing factor (OIF) on ovulation in female camels. Seminal plasma was fractionated using gel filtration chromatography, and two protein peaks were obtained and analysed by western blotting. The effect of the bioactive protein fraction (OIF/β-NGF) was tested by intramuscular injection (1 ml) in synchronized females with the following treatments: PBS (negative control group, n = 3); 20 µg Buserelin (GnRH analogue, positive control group; n = 3); and purified OIF with doses of 1 and 2 mg (n = 5, each treatment). Blood samples were collected every two days from day 0 until 14 days post-treatment, and the progesterone concentration was assessed. The obtained results showed that the OIF is highly present in the seminal plasma of dromedary camels as a protein with a molecular mass of approximately 14 kDa. It was detected as a beta-Nerve Growth Factor, named Cam-β-NGF. The effect of this molecule on ovulation was clearly demonstrated by the significant increase of the plasma progesterone concentration in the treated female groups (1 and 2 mg of Cam-β-NGF) as observed in Buserelin group. In summary, intramuscular injection of β-NGF isolated from dromedary camel seminal plasma induces ovulation in females with similar rate to Buserelin treatment.

Keywords: β-nerve growth factor, Dromedary camel, Ovulation, Seminal plasma
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How to Cite
Fatnassi, M., J. Cebrián-Pérez, I. Salhi, R. Pérez-Pé, M. Seddik, A. Casao, T. Khorchani, T. Muiño-Blanco, and M. Hammadi. “IDENTIFICATION OF β-NERVE GROWTH FACTOR IN DROMEDARY CAMEL SEMINAL PLASMA AND ITS ROLE IN INDUCTION OF OVULATION IN FEMALES”. Emirates Journal of Food and Agriculture, Vol. 29, no. 4, Feb. 2017, pp. 293-9, doi:https://doi.org/10.9755/ejfa.2016-11-1585. Accessed 15 Aug. 2020.
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Regular Articles