EVALUATION OF ROSELLE BASED ON THE INTERNAL TRANSCRIBED SPACER SEQUENCE OF RRNA GENE

Authors

  • Se Hee Park Chonnam National University, School of Biological Sciences & Technology, Gwangju, Korea;
  • Florence C. Ginibun 2 University of Malaya, Faculty of Science, Institute of Biological Sciences, Genetics and Molecular Biology Unit, Kuala Lumpur, Malaysia;
  • Mark S. Roh Dankook University, School of Bio‑Resource Science, Laboratory of Floriculture and Plant Physiology, Cheonan, Chungnam, Korea
  • Ming-Xi Li Dankook University, School of Bio‑Resource Science, Laboratory of Floriculture and Plant Physiology, Cheonan, Chungnam, Korea
  • Jeung Keun Suh Dankook University, School of Bio‑Resource Science, Laboratory of Floriculture and Plant Physiology, Cheonan, Chungnam, Korea
  • Young Hee Joung Chonnam National University, School of Biological Sciences & Technology, Gwangju, Korea;

DOI:

https://doi.org/10.9755/ejfa.2015.04.070

Keywords:

Key words: Hibiscus sabdariffa, ITS 1, 2 regions, single nucleotide polymorphism (SNP)

Abstract

Genetic differences in 5-lobed leaf Roselle (Hibiscus sabdariffa L.) collected from China, Malaysia, Sudan, and USA were evaluated by the sequence of nuclear internal transcribed spacer 1, 2 regions in rRNA gene (ITS 1, 2 regions). A total of 27 samples were subjected to polymerization chain reaction (PCR) and 729 bp bands were amplified in all samples. Samples were divided into 4 groups based on the single nucleotide polymorphisms at 71, 91, and 249 of the 729 bp band. However, no variations among samples collected from different geographic origins of H. sabdariffa are observed.

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Published

2015-05-04

How to Cite

Park, S. H., F. C. Ginibun, M. S. Roh, M.-X. Li, J. K. Suh, and Y. H. Joung. “EVALUATION OF ROSELLE BASED ON THE INTERNAL TRANSCRIBED SPACER SEQUENCE OF RRNA GENE”. Emirates Journal of Food and Agriculture, vol. 27, no. 8, May 2015, pp. 659-61, doi:10.9755/ejfa.2015.04.070.

Issue

Section

Short Communication