Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

  • Rania Benjamaa Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.
  • Abdelkarim Moujanni Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco. ; National Office of Food Safety (ONSSA), Avenue Hadj Ahmed Cherkaoui, Agdal - Rabat – Maroc.
  • Anass Terrab Department of Plant Biology and Ecology, University of Seville, Ap. 1095, 41080 Sevilla, Spain.
  • Rabiaa Eddoha Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.
  • Maryam Benbachir Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.
  • Abderrahman Moujahid Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.
  • Boubker Nasser Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.
  • Sami Darkaoui Division of Pharmacy and Veterinary Inputs, Control and Expertise Department, ONSSA, Rabat, Morocco.
  • Nadia Zyate Division of Pharmacy and Veterinary Inputs, Control and Expertise Department, ONSSA, Rabat, Morocco.
  • Ali Talmi Division of Pharmacy and Veterinary Inputs, Control and Expertise Department, ONSSA, Rabat, Morocco.
  • Abdel Khalid Essamadi Hassan First University of Settat, Faculty of Sciences and Technologies, Laboratory of Biochemistry, Neurosciences, Natural Resources and Environment, 577, Settat, Morocco.

Abstract

Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

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Benjamaa, R., A. Moujanni, A. Terrab, R. Eddoha, M. Benbachir, A. Moujahid, B. Nasser, S. Darkaoui, N. Zyate, A. Talmi, and A. K. Essamadi. “Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco”. Emirates Journal of Food and Agriculture, Vol. 32, no. 11, Nov. 2020, pp. 795-07, doi:https://doi.org/10.9755/ejfa.2020.v32.i11.2190. Accessed 28 July 2021.
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