EXTRACTIVE SUBSTANCES OF FRUIT BODY GOLDEN CHANTERELLE (CANTHARELLUS CIBARIUS FR.) AND HEDGEHOG MUSHROOM (HYDNUM REPANDUM FR.)
The purpose of this work was to perform a comparative study of the chemical composition of Cantharellus cibarius Fr. and Hydnum repandum Fr. and the revision of the data in the occurrence of carotenoids, ergocaliferol, tocopherols and phenolic compounds.
Substances extracted with 80% ethanol from dried fruit bodies of both fungi were investigated. The fatty acids quantitative and qualitative contents were examined by Gas chromatography Mass Spectrometry (GC-MS).
Lipophilic substances of hexane extract were investigated by chemical reactions and Thin-layer chromatography (TLC) after the separation of compounds from this fraction on a silica gel column.
It was determined that the hexane fraction of both mushrooms contained a large amount of higher fatty acids and their esters. Linoleic acid (31.42 %) and oleic acid (57.89 %) were the dominant components in Cantharellus cibarius and Hydnum repandum accordingly.
Tocopherols, carotenoids and ergocalciferol were also found in the hexane extracted fraction. The estimated content of tocopherols was ≈0.82% of the total mass of ethanolic extract for chanterelle and ≈0.22% for hedgehog mushroom, respectively.
Chloroform and ethyl acetate fractions did not contain tocopherols, carotenoids and ergocalciferol and their mass was insignificant.
Carbohydrates content was ≈ 54% and ≈ 49 % of the total mass of ethanolic extract and the content of aminoacids was found as ≈ 4.5% and ≈ 4.7% for Cantharellus cibarius and Hydnum repandum accordingly.
Flavonoids, phenolic substances and hydroxycinnamic acids were not found in the fractions of ethanol extracts of both mushrooms.
Barros L.; Cruz T.; Baptista P.; Estevinho LM; Ferreira IC. 2008. Wild and commercial mushrooms as source of nutrients and nutraceuticals. Food Chem Toxicol. 46(8): 2742–2747.
Barros L., Duenas M., Ferreira ICFR, Baptista, P., Santos-Buelga C. 2009. Phenolic acids determination by HPLC-DAD-ESI/MS in sixteen different Portuguese wild mushrooms species. Food Chem Toxicol 47: 1076– 1079.
Botineştean C.; Hădărugă NG.; Hădărugă DI.; Jianu I. 2012 Fatty Acids Composition by Gas Chromatography –Mass Spectrometry (GC-MS) and most important physicalchemicals parameters of Tomato Seed Oil Journal of Agroalimentary Processes and Technologies. 18(1), 89-94.
Davolia P., Muccia A., Schenettia L., Weber RWS 2005. Laetiporic acids, a family of non-carotenoid polyene pigments from fruit-bodies and liquid cultures of Laetiporus sulphureus (Polyporales, Fungi). Phytochemistry 66 (7): 817-823
Ebrahimzadeh M.A., Safdari Y., Khalili M. 2015. Antioxidant Activity of Different Fractions of Methanolic Extract of the Golden Chanterelle Mushroom Cantharellus cibarius (Higher Basidiomycetes) from Iran. Int. J. Med.Mushrooms 17(6): 557- 565.
Kakac В, Vejdelek ZJ 1974. Handbuch der photometrischen Analyse organischer Verbindungen, Bd 1-2, Prague.
Khalili M, Ebrahimzadeh MA, Omran IF, Karami M. 2014. Antihypoxic Activities of the Golden Chanterelle Mushroom, Cantharellus cibarius (Higher Basidiomycetes). Internat J Med Mushrooms, 16(4): 339–344
Kozarski M, Klaus A, Vunduk J, Zizak Z, Niksic M; Jakovljevic D, Vrvic MM, Van Griensven LJLD. 2015. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms. Food Funct., 6, 1875-1886
Long Chen, Xichun Peng, Jiaying Lv, Siyin Liao, Shiyi Ou, Yingbin Shen. 2017. Purification and Structural Characterization of a Novel Water-Soluble Neutral Polysaccharide from Cantharellus cibarius and Its Immunostimulating Activity in RAW264.7 Cells. Internat J Polymer Science. Article ID 3074915, 9 pages https://doi.org/10.1155/2017/3074915
Makarenko O.A., Levitsky A.P. 2013. Physiological functions of flavonoids in plants. Physiol., Biochem. Cult. Plants. 45(2): 100 -112 (in Russian).
Mathesius U. 2018. Flavonoid Functions in Plants and Their Interactions with Other Organisms. Plants 7: 30-33; doi:10.3390/plants7020030
Methods of carbohydrate chemistry. transl. from English / ed. N.K. Kochetkova 1967. Moscow: Mir 512 (in Russian)
Mui D, Feibelmant T, Bennett JW 1998. A preliminary study of the carotnoids of some north american species of cantharellus. Int. J. Plant Sci. 159(2): 244-248.
Nwofor SC, Morenikeji OA, Morenike AIO, Oyeyemi OT 2019. Inhibitory activities of ethanolic extracts of two macrofungi against eggs and miracidia of Fasciola spp. Open Life Sciences 13(1): 504-510.
Pochinok H.N. 1976. Methods of biochemical analysis of plants. Kiev: Naukova Dumka., 334 p. (in Russian).
Stryamets N.; Elbakidze M.; Ceuterick M.; Angelstam P.; Axelsson R. 2015. From economic survival to recreation: contemporary uses of wild food and medicine in rural Sweden, Ukraine and NW Russia. Journal of Ethnobiology and Ethnomedicine 11: 53
Tsyganov MA, Vishnivetskaya GB, Kukina TP, Sorokina IV, Lvova MN, Protsenko MA, Kostina NE, Avgustinovich DF 2018. Studyng the effects of Cantharellus cibarius fungi on Opisthorchis felineus trematode and on parasite host – c57Bl/6 inbred mice. Vavilov Journal of Genetics and Breeding 22(7): 850-858. DoI 10.18699/VJ18.426 (in Russian)
Valentao P, Andrade PB, Rangel J, Ribeiro B, Silva BM, Baptista P, Seabra RM.2005. Effect of the conservation procedure on the contents of phenolic compounds and organic acids in chanterelle (Cantharellus cibarius) mushroom. J. Agric. Food. Chem. 53(12): 4925–4931
Vamanu E, Nita S. 2014. Bioactive Compounds, Antioxidant and Anti-inflam¬matory Activities of Extracts from Cantharellus cibarius. Rev. Chim. (Bucharest). 65(3): 372 -379.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.