EXTRACTIVE SUBSTANCES OF FRUIT BODY GOLDEN CHANTERELLE (CANTHARELLUS CIBARIUS FR.) AND HEDGEHOG MUSHROOM (HYDNUM REPANDUM FR.)

Volodymyr Antonyuk; Lidiia V. Panchak; Lidiia Ya Antonyuk; Alina R. Zyn

doi:10.9755/ejfa.2020.v32.i11.2195

Authors

Volodymyr Antonyuk Department of inorganic and bioinorganic chemistry of Danylo Halytsky Lviv National Medical University; Pekarska str., 69, Lviv, Ukraine
Lidiia V. Panchak Lviv Medical College of Postgraduate Education, Mykolaichuka str., 9, Lviv, Ukraine.
Lidiia Ya Antonyuk Lviv Research Forensic Centre of MIA of Ukraine, Koniushynna str., 24, Lviv, Ukraine.
Alina R. Zyn Department of inorganic and bioinorganic chemistry of Danylo Halytsky Lviv National Medical University; Pekarska str., 69, Lviv, Ukraine; Institute of Cell Biology, National Academy of Sciences of Ukraine, 14/16 Dragomanova str., Lviv, Ukraine

DOI:

https://doi.org/10.9755/ejfa.2020.v32.i11.2195

Abstract

The purpose of this work was to perform a comparative study of the chemical composition of Cantharellus cibarius Fr. and Hydnum repandum Fr. and the revision of the data in the occurrence of carotenoids, ergocaliferol, tocopherols and phenolic compounds.

Substances extracted with 80% ethanol from dried fruit bodies of both fungi were investigated. The fatty acids quantitative and qualitative contents were examined by Gas chromatography Mass Spectrometry (GC-MS).

Lipophilic substances of hexane extract were investigated by chemical reactions and Thin-layer chromatography (TLC) after the separation of compounds from this fraction on a silica gel column.

It was determined that the hexane fraction of both mushrooms contained a large amount of higher fatty acids and their esters. Linoleic acid (31.42 %) and oleic acid (57.89 %) were the dominant components in Cantharellus cibarius and Hydnum repandum accordingly.

Tocopherols, carotenoids and ergocalciferol were also found in the hexane extracted fraction. The estimated content of tocopherols was ≈0.82% of the total mass of ethanolic extract for chanterelle and ≈0.22% for hedgehog mushroom, respectively.

Chloroform and ethyl acetate fractions did not contain tocopherols, carotenoids and ergocalciferol and their mass was insignificant.

Carbohydrates content was ≈ 54% and ≈ 49 % of the total mass of ethanolic extract and the content of aminoacids was found as ≈ 4.5% and ≈ 4.7% for Cantharellus cibarius and Hydnum repandum accordingly.

Flavonoids, phenolic substances and hydroxycinnamic acids were not found in the fractions of ethanol extracts of both mushrooms.

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