Effect of nutrient solution concentration on phenology, stevioside content, total phenolic compounds and total flavonoids in leaves of Stevia rebaudiana Bertoni

Karla Rubí Jiménez Sánchez; Adán Mercado Luna; Ana Angélica Feregrino Pérez; Juan Fernando García Trejo; Jorge Luis Chávez Servín; Reynaldo Hernández Maldonado; Karla Nicol Hernández Puente; Carlos Alberto Murillo Cardenas

doi:10.9755/ejfa.2021.v33.i3.2666

Authors

Karla Rubí Jiménez Sánchez Departamento de Biosistemas, Facultad de Ingeniería, Centro de Investigación y Desarrollo Tecnológico en Materia Agrícola, Pecuaria, Acuícola y Forestal (CIDAF), Universidad Autónoma de Querétaro, Campus Amazcala. Carretera a Chichimequillas S/N, Amazcala, El Marqués, Querétaro 76130, Mexico.
Adán Mercado Luna Departamento de Biosistemas, Facultad de Ingeniería, Centro de Investigación y Desarrollo Tecnológico en Materia Agrícola, Pecuaria, Acuícola y Forestal (CIDAF), Universidad Autónoma de Querétaro, Campus Amazcala. Carretera a Chichimequillas S/N, Amazcala, El Marqués, Querétaro 76130, Mexico.
Ana Angélica Feregrino Pérez Departamento de Biosistemas, Facultad de Ingeniería, Centro de Investigación y Desarrollo Tecnológico en Materia Agrícola, Pecuaria, Acuícola y Forestal (CIDAF), Universidad Autónoma de Querétaro, Campus Amazcala. Carretera a Chichimequillas S/N, Amazcala, El Marqués, Querétaro 76130, Mexico.
Juan Fernando García Trejo Departamento de Biosistemas, Facultad de Ingeniería, Centro de Investigación y Desarrollo Tecnológico en Materia Agrícola, Pecuaria, Acuícola y Forestal (CIDAF), Universidad Autónoma de Querétaro, Campus Amazcala. Carretera a Chichimequillas S/N, Amazcala, El Marqués, Querétaro 76130, Mexico.
Jorge Luis Chávez Servín Facultad de Ciencias Naturales, Campus Juriquilla, Universidad Autónoma de Querétaro. Av. de las Ciencias S/N, Juriquilla, Querétaro 76320, Mexico.
Reynaldo Hernández Maldonado Facultad de Ingeniería, Campus Amealco, Carretera Amealco –Temascalcingo km 1 col. Amealco, Querétaro 76850, Mexico.
Karla Nicol Hernández Puente Facultad de Ingeniería, Campus Amealco, Carretera Amealco –Temascalcingo km 1 col. Amealco, Querétaro 76850, Mexico.
Carlos Alberto Murillo Cardenas Campus Jalpan, Universidad Autónoma de Querétaro. Blvd. Policarpio Olvera s/n Frac. El Coco, Jalpan, Querétaro, 76340, Mexico.

DOI:

https://doi.org/10.9755/ejfa.2021.v33.i3.2666

Abstract

Among the most interesting areas of food technology research today is the search for natural alternatives to common sweeteners. One of these is Stevia rebaudiana Bertoni, which contains antioxidants and stevioside whose concentration can be affected by factors such as plant nutrition during its production. The objective of the study was to determine the effect of mineral nutrition levels (N, P, K Ca, Mg, S, Fe, Mn, B, Zn, Cu) in Steiner nutrient solution (SNS) on phenology and to quantify chlorophyll concentration (CC), stevioside content (SC), total phenolic compounds (TPC) and total flavonoids (TF) in the leaves of Stevia rebaudiana Bertoni. Five concentrations of SNS were evaluated: 0%, 25%, 50%, 75% and 100%. The experimental design was randomized with three replications. The experimental unit consisted of six plants per treatment (6 x 3 = 18 plants). The highest plant height (18.58 cm) was obtained with the 25% and 100% SNS concentrations; while the highest fresh weight and dry weight (21.70 ± 0.70 and 4.17 ± 0.12 g/ plant) were obtained with a 50% solution. The highest CC (41.31 ± 0.93 SPAD Units) and SC (8.88 ± 0.54 mg of stevioside / g dry matter (DM) were observed with 0% SNS. The highest contents of TF (16.42 ± 1.12 µg RE / g DM) and TPC (8.57 ± 1.12 µg GAE / g DM) were obtained with a 50% SNS solution. Finally, the compounds of interest were analyzed with respect to the total biomass obtained. The 75% SNS solution resulted in the highest amount of stevioside (17.03 mg / plant) and the second highest value for TPC (27.19 µg GAE / plant) and TF (64.57 µg RE / plant) content.

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References

Alcántar-González, G. and L. I. Trejo-Téllez. 2010. Nutrición de Cultivos. Colegio de Posgraduados and Mundi–Prensa. Mexico, D. F. 454 p. ISBN 978–968–7462–48–6.
Aranda-González, I., M. Segura-Campos, Y. Moguel-Ordoñez, D. Betancur-Ancona. 2014. Stevia rebaudiana Bertoni. Un potencial adyuvante en el tratamiento de la diabetes mellitus. J. Food. 12: 218-226.
Arias-Zabala, M., M. J. Angarita-Velásquez, A. M., Aguille-Cardona, M., Restrepo F. and C. Montoya-Vallejos. 2009. Strategies for the improvement of secondary metabolites production in plan cell cultures, Rev. Fac. Nal. Agr. Medellín 62: 4881- 4895.
Bonilla-Correa., C. R., M. S. Sánchez and D. F. Perlaza. 2007. Evaluation of propagation methods, nitrogen fertilization and phenology in stevia in the Cauca Valley, Colombia. J. Acta Agron. 56: 131-134.
Cardador-Martínez, A., G. Loarca-Piña and B. D. Oomah. 2002. Antioxidant activity of common beans (Phaseolus Vulgaris L.). J. Agric. Food Chem. 50: 6975–6980.
Clementelli, A. and R. Zevallos. 2009. Fertilización nitrogenada mineral y orgánica en el cultivo de stevia (Stevia rebaudiana Bertoni). J. Univ. Cienc. Soc. 1: 47-50.
Dewanto, V., X. Wu, K. K. Adom and R. H. Liu. 2002. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. J. Agric. Food Chem. 50: 3010–3014.
Evangelista-Lozano, S., Estrada-García, M., Escobar-Arellano, S. L., Arenas-Ocampo, M. L., Camacho-Díaz, B. H. and Jiménez-Aparicio, A. R. (2015). Eucharis grandiflora Planch. and Linden development fertirrigated with different osmotic potentials. Journal of Plant Nutrition, 38(4): 551–567
Gastelum-Osorio, D.A. Sandoval-Villa, M. Trejo-López, C. Trejo-López, R. 2013. Fuerza iónica de la solución nutritiva y densidad de plantación sobre la producción y calidad de frutos de Physalis peruviana L.. Revista Chapingo. Serie horticultura, 19(2), 197-210. https://doi.org/10.5154/r.rchsh.2012.01.002.
Jarma, A., M. Espitia, and G, Fischer. 2010. Síntesis de esteviósidos enestevia (Stevia rebaudiana Bert.). Acta Biológica Colombiana, 15(1):263-268
Jarma-Orozco, A., E.M. Combatt and J. Polo. 2012. Glycoside contents depending on the nutrient deficiencies in Stevia rebaudiana Bert. J. U. D. C. A. Act. & Div. Cient. 15: 107–116.
Krugh, B., L. Bickam and D. Miles. 1994. The solid-state chlorophyll meter, a novel instrument for rapidly and accurately determining the chlorophyll concentrations in seedling leaves. J. Maize Genetics Cooperation Newsletter. 68: 25-27.
Garcia-Mier, L. A. E. Meneses-Reyes, S. N. Jimenez-Garcia, A. M. Luna-, J. F. G. Trejo, L. M. Contreras-Medina, A. A. Feregrino-Perez , " Polyphenol Content and Antioxidant Activity of Stevia y menta como resultado de la fertilización orgánica y convencional ", Journal of Food Quality , vol. 2021 , ID de artículo 6620446 , 6 páginas , 2021 . https://doi.org/10.1155/2021/6620446
Rao, G. N., P. R. Pamidighantam, K. Balaswamy and S. Akula. 2014. Antioxidant activity of stevia (Stevia rebeudiana L.) leaf powder and a commercial stevioside powder. J. Food Pharm. Sci. 2: 32-38.
Oomah, B.D., A. Cardador-Martínez, G. Loarca-Piña. 2005. Phenolics and antioxidative activities in common beans (Phaseolus vulgaris L). J. Sci. Food and Agric. 85: 935–942.
Pal, P. K., R. Kumar, V. Guleria, M. Mahajan, R. Prasad, V. Pathania, B. S. Gill, D. Singh, G. Chand, B. Singh, R. D. Singh and P. S. Ahuja. 2015. Crop-ecology and nutritional variability influence growth and secondary metabolites of Stevia rebaudiana Bertoni. J. BMC Plant Biol. 15: 1-16.
Ramirez, G. 2011. Paquete tecnológico Estevia (Stevia rebaudiana) Establecimiento y mantenimiento. Programa estratégico para el desarrollo rural sutentable de la región sur -sureste de México: Tropico Húmedo,1–15.
Romero-Figueroa J. C., M. C. Gutiérrez-Castorena, C. B. Peña-Valdivia, M. N. Rodríguez-Mendoza, J. A. Escalante-Estrada, J. A. Cueto-Wong and E. Burguete-Hernández. 2017. Growth dynamics of Stevia cultivated in organic substrates in greenhouse. J. Fitotec. Mex. 40: 341–350.
Soufi, S., S. Aabaoui, S. Jelleli, R. Salah and T. Bettaieb. 2019. High Photochemical Efficiency and Nutrient Homeostasis in Stevia (Stevia rebaudiana) Plant Leaf Increases Tolerance to Saline Irrigation water. J. Agric. & Biol. 22: 1059–1064.
Steiner, A. 1984. The Universal Nutrient Solution, Proceedings of IWOSC 1984 6th International Congress on Soilless Culture, pp. 633-650, Wageningen, The Netherlands.
Tavarini, S., C. Sgherri, A. M. Ranieri and L. G. Angelini. 2015. Effect of Nitrogen Fertilization and Harvest Time on Steviol Glycosides, Flavonoid Composition, and Antioxidant Properties in Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry, 63(31):7041–7050.
Utumi, M., P. Monnerat, P. Gomes-Pereira, P. Rezende-Fontes and V. Campos-Godinho. 1999. Deficiência de macronutrientes em estévia: sintomas visuais e efeitos no crescimento, composição química e produção de esteviosídeo. J. Pesq. Agropec. Bras. 34: 1039-1043.
Vázquez-Baxcajay, L., A. Robledo-Paz, A. Muratalla-Lúa and V. Conde-Martínez. 2014. Micropropagación de Stevia rebaudiana Bertoni y detección de steviósidos. J. Bioagro. 26: 49–56.
Zhang, Q., H. Yang, L. Yongning, H. Liu and X. Jia. 2017. Toxicological evaluation of ethanolic extract from Stevia rebaudiana Bertoni leaves: Genotoxicity and subchronic oral toxicity. J. Sci. Direct. 86: 253-259.