Chemical and productivity characterization of parental and hybrid strains of Lentinula edodes cultivated in different agricultural residues

  • Juan Diego Valenzuela Cobos Facultad de Ciencias e Ingeniería, Universidad Estatal de Milagro (UNEMI) - Ecuador.
  • Fabricio Guevara Viejó Facultad de Ciencias e Ingeniería, Universidad Estatal de Milagro (UNEMI) - Ecuador.
  • Jesennia Cárdenas Cobo Facultad de Ciencias e Ingeniería, Universidad Estatal de Milagro (UNEMI) - Ecuador.
  • Rafael Lazo Sulca Facultad de Ciencias e Ingeniería, Universidad Estatal de Milagro (UNEMI) - Ecuador.
  • Delia Noriega Verdugo Facultad de Ciencias e Ingeniería, Universidad Estatal de Milagro (UNEMI) - Ecuador.
  • María Fernanda Garcés Moncayo Facultad de Ingeniería, Escuela Ingeniería Ambiental. Universidad Nacional de Chimborazo. Km 1/vía a Guano s/n, Riobamba, 060150. Ecuador.
  • Ana Grijalva Endara Facultad de Ciencias Químicas, Universidad de Guayaquil - Ecuador.

Abstract

 


Productivity of parental and hybrid strains of Lentinula edodes cultivated with two mixtures of agricultural wastes (mixture 1: peat moss, banana leaves, oak sawdust, millet seed, cotton seed hull and CaCO3, and mixture 2: wheat straw, oak sawdust, cotton seed hull and CaCO3), the chemical composition of the fruit bodies and also the antioxidant activities of hexanic extracts of the mushrooms were evaluated. The strains of Lentinula edodes (LEN97 and LEN954XLEC241) cultivated on both mixtures produced fruit bodies with highest protein contents since 16.71 to 17.10%. The hybrid strain of Lentinula edodes strain (LEN954XLEC241) cultivated on both mixtures showed the highest biological efficiencies since 74.61 to 82.80% and production rates between 1.21 and 1.30%, and also their hexanic extracts presented the highest antioxidant effects ranged from 8.01 to 9.84 mg mL- 1. The use of hybrid strain of Lentinula edodes can be used to improve the production of this genera.

References

AOAC. (2005). Official Methods of Analysis. 18th ed. Association Analytical Chemists, Gaithersburg, Maryland, USA.
Bach, F., C. Vieira Helm, E. Alves De Lima, M.B. Bellettini and C.W.I. Haminiuk. 2018. Influence of cultivation methods on the chemical and nutritional characteristics of Lentinula edodes. Emir. J. Food Agric. 30: 1006-1013.
Brisko, N.A., V.G. Babitskay, V.T. Bilay and N.Y. Mitropolskaya. 2002. Biologically active substances from Lentinula edodes and Pleurotus ostreatus. In: Sánchez JE, Huerta G, Montiel E (eds) Proceedings of the Fourth International Conference on Mushroom Biology and Mushroom Products. UAEM, México, pp 383–389.
Cabrera, R., M. Valenzuela, Y. Reyes, M. Esqueda and A. Asaff. 2013. Caracterización de los extractos metanólicos de poda de vid y paja de trigo y su oxidación por enzimas extracelulares de Lentinula edodes. Rev. Mex. Micol. 37: 61-67.
Chang, S.T. and P.G. Miles. 2004. Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. CRC Press, Boca Ratón.
Diallo, I., F. Boudard, S. Morel, M. Vitou, C. Guzman, N. Saint, A. Michel, S. Rapior, L. Traoré, P. Poucheret and F. Fons. 2020. Antioxidant and anti-inflammatory potential of Shiitake culinary-medicinal mushroom, Lentinus edodes (Agaricomycetes) sporophores from various culture conditions. Int. J. Med. Mushrooms. 22: 535-46.
Gaitán-Hernández, R., M. Esqueda, A. Gutiérrez, A. Sánchez, M. Beltrán-García and G. Mata. 2006. Bioconversion of agrowastes by Lentinula edodes: The high potential of viticulture residues. Appl. Microbiol. Biotechnol. 71: 432-439.
Gaitán-Hernández, R., M. Esqueda, A. Gutierrez and M. Beltrán-García. 2011. Quantitative changes in the biochemical composition of lignocellulosic residues during the vegetative growth of Lentinula edodes. Braz. J. Microbiol. 42: 30-40.
Gaitán-Hernández, R., N. Cortés and G. Mata. 2014. Improvement of yield of the edible and medicinal mushroom Lentinula edodes on wheat straw by use of supplemented spawn. Braz. J. Microbiol. 45: 467–474.
Gao, S., Z. Huang, X. Feng, Y. Bian, W. Huang and Y. Liu. 2020. Bioconversion of rice straw agro-residues by Lentinula edodes and evaluation of non-volatile taste compounds in mushrooms. Sci. Rep. 10: 1814.
Harris-Valle, C., M. Esqueda, A. Sánchez, M. Beltrán-García and E.M. Valenzuela-Soto. 2007. Polar vineyard pruning extracts increase the activity of the main ligninolytic enzymes in Lentinula edodes cultures. Can. J. Microbiol. 53: 1150–1157.
Kitzberger, C.S.G., A.Jr. Smania, R.C. Pedrosa and S.R.S. Ferreira. 2007. Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids. J. Food Eng. 80: 631–638.
Lin, F.C., X.M. Yang and Z.W. Wang. 2000. Cultivation of the black oak mushroom Lentinula edodes in China. Mushroom Sci.5: 955-958.
Mocan, A., A. Fernandes, L. Barros, G. Crişan, M. Smiljković, M. Soković and I.C.F. Ferreira. 2018. Chemical composition and bioactive properties of the wild mushroom Polyporus squamosus (Huds.) Fr: A study with samples from Romania. Food Sci. Nutr. 9: 160-170.
Plotnikov, EV, L.B. Glukhova, L.O. Sokolyanskaya, O.V. Karnachuk and M. Solioz. (2016. Effect of tree species on enzyme secretion by the shiitake medicinal mushroom, Lentinus edodes (Agaricomycetes). Int. J. Med. Mushrooms 18(7):637-644.
Przybylowicz, P. and J. Donoghue. 1990. Shiitake Growers Handbook, The Art and science of Mushroom cultivation. Kendall/Hunt Publishing Company. ISBN 0-84034962-9.
Reguła, J. and M. Siwulski. 2007. Dried shiitake (Lentinulla edodes) and oyster (Pleurotus ostreatus) mushrooms as a good source of nutrient. Acta Sci. Pol. Technol. Aliment. 6: 1644–0730.
Royse, D.J., J. Baars, Q. Tan. Current Overview of Mushroom Production in the World. In Edible and Medicinal Mushrooms: Technology and Applications; D.C. Zied, A. Pardo-Gimenez, A., Eds.; John Wiley & Sons Ltd.: Chichester, UK, 2017; pp. 5–13.
Salmones, D., R. Gaitán-Hernández, R. Pérez and G. Guzmán. 1997. Estudios sobre el género Pleurotus VIII. Interacción entre crecimiento micelial y productividad. Rev. Iberoam. Micol. 14: 173-176.
Sánchez-Hernández, A., G.V. Toro, H. Leal-Lara, M.E. Garín-Aguilar, R. Villanueva-Arce and R. Ramírez-Carrillo. 2014. Lentinula edodes dedikariotization and production of inter-generic hybrids. J. Chem. Biol. Phys. Sci. 4: 130.
Sharma, V.P., S. Kamal, R.C. Upadhyay, S. Kumar, S.K. Sanyal and M. Singh. 2015. Taxonomy, phylogeny, cultivation and biological activities of a Lentinus species from Andaman and Nicobar Islands (India). Emir. J. Food Agric. 27: 570-576.
Silva, E.M., A. Machuca and A.M.F. Milagres. 2005. Effects of cereal brans on Lentinula edodes growth and enzyme activities during cultivation on forestry wastes. Lett. Appl. Microbiol. 40: 283–8
Valenzuela-Cobos, J.D., E. Durán Páramo, R. Villanueva Arce, A. Sánchez Hernández, M.E. Garín Aguilar, H. Leal Lara and G. Valencia del Toro. 2017. Production of hybrid strains among Pleutorus and Lentinula and evaluation of their mycelial growth kinetics on malt extract agar and wheat grain using the Gompertz and Hill models. Emir. J. Food Agric. 29: 927-935.
Valenzuela-Cobos, J.D., G. Vásquez-Véliz, D.C. Zied, O.M. Franco-Hernández, A. Sánchez Hernández, M.E. Garín Aguilar, H. Leal Lara and G. Valencia del Toro. 2019a. Bioconversion of agricultural wastes using parental, hybrid and reconstituted strains of Pleurotus and Lentinula. Rev. Mex. Ing. Quím. 18: 647-657.
Valenzuela-Cobos, J.D., R.O. Rodríguez-Grimón, D.C. Zied, A. Grijalva-Endara, M.F. Garcés-Moncayo, M.E. Garín-Aguilar, A. Sánchez-Hernandez and G. Valencia del Toro. 2019b. Chemical composition and biological properties of Pleurotus spp. cultivated on different concentrations of peat moss and wheat straw. Emir. J. Food Agric. 31: 830-836.
Valenzuela-Cobos, J.D., A. Grijalva-Endara, R. Marcillo-Vallejo and M.F. Garcés-Moncayo, M.F. 2020. Production and characterization of reconstituted strains of Pleurotus spp. cultivated on different agricultural wastes. Rev. Mex. Ing. Quím. 19: 1493-1504.
Xu, S., F. Wang, Y. Fu, D. Li, X. Sun, C. Li, B. Song and Y. Li. 2020. Effects of mixed agro-residues (corn crop waste) on lignin-degrading enzyme activities, growth, and quality of Lentinula edodes. RSC Adv. 10: 9798-9807.
Statistics
54 Views | 82 Downloads
How to Cite
Cobos, J. D. V., F. G. Viejó, J. C. Cobo, R. L. Sulca, D. N. Verdugo, M. F. G. Moncayo, and A. G. Endara. “Chemical and Productivity Characterization of Parental and Hybrid Strains of Lentinula Edodes Cultivated in Different Agricultural Residues”. Emirates Journal of Food and Agriculture, Vol. 33, no. 3, Apr. 2021, pp. 260-5, doi:https://doi.org/10.9755/ejfa.2021.v33.i3.2668. Accessed 24 June 2021.
Section
Short Communication