Physiological and physicochemical behavior of guamara (Bromelia pinguin) and cocuixtle (Bromelia karatas) fruits, as well as the antibacterial effect of their pre-purified proteases
DOI:
https://doi.org/10.9755/ejfa.2021.v33.i4.2686Abstract
The objective was to study the behavior of the respiration rate (RR) of guamara and cocuixtle fruits during storage, the effect or the maturity stage (green maturity and consumption maturity) of these fruits on physicochemical parameters and specific proteolytic activity, as well as the antibacterial effect of the prepurified proteases of these fruits. The guamara and cocuixtle fruits presented a RR of 15 mL CO2/kg·h and 10 mL CO2/kg·h, respectively; and because the ethylene production was not detected in any of the species, it was concluded that both fruits are of the non-climacteric type. The cocuixtle fruits presented the highest specific enzymatic activity in the state of green maturity and the guamara fruits in the state of consumption maturity, with 18.99 and 53.88 U/mg of protein, respectively. Likewise, pre-purified proteases from both fruits showed antibacterial activity against E. coli and S. aureus. It is concluded that ripened guamara fruits and green cocuixtle fruits can be an important source of proteases for the food industry and they can be used against pathogens.
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Eshamah, H., Han, I., Naas, H., Rieck, J. and Dawson, P. 2013. Bactericidal effects of natural tenderizing enzymes on Escherichia coli and Listeria monocytogenes. J. Food Res 2(1): 8-18. Doi:10.5539/jfr.v2n1p8
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Liu, M., Pirrello, J., Chervin, C., Roustan, J. P. and Bouzayen, M. 2015. Ethylene control of fruit ripening: revisiting the complex network of transcriptional regulation. Plant Physiol. 169(4): 2380–2390. Doi:10.1104/pp.15.01361
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Looby, C., Hauge, J. B., Barry, D. and Eaton, W. D. 2012. Fungal inhibition by Bromelia pinguin (Bromeliaceae) and its effect on nutrient cycle dynamics. Trop. Ecol. 53(2): 225–234.
López, L. M., Sequeiros, C., Natalucci, C. L., Brullo, A., Maras, B., Barra, D. and Caffini, N. O. 2000. Purification and Characterization of Macrodontain I, a Cysteine Peptidase from Unripe Fruits of Pseudananas macrodontes (Morr.) Harms (Bromeliaceae). Protein Expr. Purif. 18(2): 133–140. Doi: 10.1006prep.1999.116
Lugo-Vargas, A. F., Tarra-Jaramillo, D., Nieto-Guzmán, M. N. and Maldonado-Rodríguez, J. 2016. Caracterización fitoquímica del extracto hidroalcohólico del fruto de Bromelia pinguin y evaluación de su actividad antifúngica frente a un hongo aislado de fruto de cacao (Theobroma cacao L.) en estado de pudrición. RIUQ. 28(2): 49–55. Doi: 10.33975/riuq.vol28n2.14
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Published
2021-05-21
How to Cite
Efigenia, M.-G., A.-E. L. Miguel, M.-O. A. O. A. Pay, S.-B. J. Alberto, and G.-M. M. de Lourdes. “Physiological and Physicochemical Behavior of Guamara (Bromelia Pinguin) and Cocuixtle (Bromelia Karatas) Fruits, As Well As the Antibacterial Effect of Their Pre-Purified Proteases”. Emirates Journal of Food and Agriculture, vol. 33, no. 4, May 2021, pp. 277-86, doi:10.9755/ejfa.2021.v33.i4.2686.
Issue
Section
Research Article
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