Mapping and validation of markers linked to Rf gene in sweet pepper (Capsicum annuum var grossum)

  • Manoj Kumar Nalla Division of Vegetable Science, ICAR-IARI, New Delhi 110012. 2Division of Germplasm Evaluation, ICAR-NBPGR, New Delhi 110012.
  • Vinod Kumar Sharma Division of Vegetable Science, ICAR-IARI, New Delhi 110012. 2Division of Germplasm Evaluation, ICAR-NBPGR, New Delhi 110012.
  • Vinukonda Rakesh Sharma Division of Vegetable Science, ICAR-IARI, New Delhi 110012. 2Division of Germplasm Evaluation, ICAR-NBPGR, New Delhi 110012.

Abstract

Cytoplasmic male sterility (CMS), is the most valuable system in commercial hybrid seed production in hot pepper. Whereas in sweet pepper (Capsicum annuum var grossum), the unavailability of a stable restorer’s line is one of the major constraints in commercial hybrid breeding. Identifying the markers linked to CMS and nuclear restorer-of-fertility (Rf) genes will help in the production of CMS hybrids in sweet pepper. In the present study, we identified seven markers co-segregating with Rf genes using bulk segregating analysis (BSA) on the F2 Rf-segregating mapping population. Among these, the CRF-SCAR marker proved to be linked to the fertility restorer gene and it could be used for screening of genotypes to identify restorers and non-restorer lines in future Capsicum breeding programs and help in the establishment of CMS system for commercial hybrid seed production.

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How to Cite
Nalla, M. K., V. K. Sharma, and V. R. Sharma. “Mapping and Validation of Markers Linked to Rf Gene in Sweet Pepper (Capsicum Annuum Var Grossum)”. Emirates Journal of Food and Agriculture, Vol. 33, no. 6, Aug. 2021, pp. 443-9, doi:https://doi.org/10.9755/ejfa.2021.v33.i6.2717. Accessed 2 Dec. 2022.
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Research Article