Chemical composition and antioxidant, antibacterial and cytotoxic properties of essential oil from Teucrium polium L. from Riyadh province

  • Waad A. Al-Otaibi Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia
  • Sahar M. AlMotwaa Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia

Abstract

This study was carried out to extract essential oil from the aerial parts of Teucrium polium L. (TPO) using hydro-distillation, and to determine
the chemical composition of the extract with GC-MS analysis. The plant was obtained from Huraymila City in the North-Western Riyadh. Total
contents of phenolics (TPC) and flavonoids (TFC) were estimated as gallic acid (GA) equivalent and catechin (CH) equivalent, respectively,
whereas the antioxidant activity was measured with DPPH assay. Agar disc diffusion and micro-broth dilution methods were utilized for
antibacterial evaluation, while MTT assay was used for cytotoxicity evaluation. The average yield of TPO was 0.26±0.01%(v: w), and the
predominant constituents were τ-cadinol (25.58%), α-fenchene (20.09%), β-eudesmol (11.76%), β-myrcene (8.02%), γ-cadinene (5.22%),
α-phellandrene (3.11%), epi-bicyclosesquiphellandrene (3.07%), and caryophyllene oxide (2.48%), all of which accounted for 79.33% of
the identified compounds. The TPC and TFC of TPO were 138.33±3.1 µg GA equivalents mL−1 and 19±2.15 µg CH equivalents mL−1,
respectively. The oil had good radical-scavenging activity, with IC50 of 61.38± 4.33 µg mL−1. It exhibited antibacterial activity against
Gram-positive S. aureus and MRSA at 16.67 % (v/v), with inhibition zones of 13± 0.09 and 11± 0.13 mm, respectively, and MIC: MBC
values of 0.26/0.52 and 0.5/21.04, respectively. However, it had no effect on the growth of negative strains (S. sonnei, P. aeruginosa,
K. pneumoniae and E. coli). Results of anti-proliferative effects of TPO on two human colon cancer cell lines (HT-29 and HCT116) and
hepatic cancer HepG2 cells, showed that it had a more cytotoxic effect on HT-29 and HCT116 cells, with IC50 values of 0.07± 0.03 and
0.14±0.06, respectively than on HepG2 which was markedly inhibited only at higher concentrations (p< 0.001). In conclusion, TPO
exerted antimicrobial and antiproliferative properties. Overall, these findings are considered useful addition to biodiversity data and may
be useful in food biotechnology and pharmaceutical industries.
Keywords: Herbal Medicine; Gas Chromatography-Mass Spectrometry; Teurium Polium L.; Antibacterial Activity; Anticancer Activity;
Essential Oil.

Author Biographies

Waad A. Al-Otaibi, Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia

Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia

Sahar M. AlMotwaa, Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia

Department of Chemistry, College of Science and Humanities, Shaqra University, Saudi Arabia

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How to Cite
Al-Otaibi, W. A., and S. M. AlMotwaa. “Chemical Composition and Antioxidant, Antibacterial and Cytotoxic Properties of Essential Oil from Teucrium Polium L. From Riyadh Province”. Emirates Journal of Food and Agriculture, Vol. 34, no. 1, Feb. 2022, doi:https://doi.org/10.9755/ejfa.2022.v34.i1.2802. Accessed 25 June 2022.
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Research Article