New insights into the mode of action of thiamethoxam on seed germination physiology


  • Iandra Rocha Barbosa ¹Crop Physiology and Metabolism Lab (LAFIMEPRO), Institute of Agricultural Sciences, Federal University of Viçosa, Campus Rio Paranaíba, Brazil
  • Danúbia Aparecida Costa Nobre Laboratory of Processed Products of Plant Origin (LPPPO), Federal University of Jequitinhonha and Mucuri Valleys, Campus Diamantina, MG, Brazil
  • Denilson Ferreira de Oliveira Natural Products Laboratory, Chemistry Department, Federal University of Lavras, Lavras, MG, Brazil
  • Geraldo Silva Laboratory for the Development of Natural Agrochemicals (LDAN), Instituto de Ciências Exatas, Universidade Federal da Universidade Federal, Campus Rio Paranaíba, MG, Brazil
  • Willian Rodrigues Macedo Crop Physiology and Metabolism Lab (LAFIMEPRO), Institute of Agricultural Sciences, Federal University of Viçosa, Campus Rio Paranaíba



In order to contribute to understanding the mode of action of the thiamethoxam in plants, we have carried out a virtual screening and
in silico molecular docking study with TIA. The pharmacophoric search for protein ligands with three-dimensional structures similar to
TIA resulted in several protein ligands with Tanimoto score equal to or greater tha 0.5, but only the ligand isoniazid (ISZ) was bound to
a protein from plant origin, which was an ascorbate peroxidase (APX). Molecular docking of ISZ and TIA to several APX’s revealed that
TIA binds more strongly to these enzymes than ISZ, suggesting that TIA acts on plants through inhibition of this enzyme. When seedlings
obtained from wheat and soybean seeds treated with different concentrations ot TIA, the activity of APX decreased with increasing TIA
concentration. Therefore, both in silico and experimental results suggest that TIA can promotes plant growth through inhibition of the
enzyme APX


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How to Cite

Rocha Barbosa, I., D. A. Costa Nobre, D. Ferreira de Oliveira, G. Silva, and W. R. Macedo. “New Insights into the Mode of Action of Thiamethoxam on Seed Germination Physiology”. Emirates Journal of Food and Agriculture, vol. 34, no. 5, June 2022, doi:10.9755/ejfa.2022.v34.i5.2857.



Research Article