Isolation and identification of phosphate solubilizing microbes in the rhizosphere of maize by sound exposure

Abstract

Phosphorus (P) is a macronutrient requirement by plants. P elements applied to the soil are quickly deposited into an insoluble form. Insoluble P in the soil can be converted into available P by phosphate solubilizing microbes. One of the factors that affect the activity and growth of phosphate solubilizing microbes is sound. This study aims to isolate and identify phosphate solubilizing soil microbes in the rhizosphere of maize by sound exposure. The research was conducted by sound treatment and without sound of Al-Quran recitation in the rhizosphere of maize, then isolated and identified the phosphate solubilizing microbes. The research results obtained 11 isolates consisting of three isolates of fungi and eight isolates of bacteria. Fungi isolates found on sound-exposure soil. Subsequently, the highest microbes of phosphate dissolution index and growth curve after the sound exposure will be identified. The results of identification using the PCR-16S rRNA sequencing method showed the phosphate solubilizing bacteria isolate TSB1 was Burkholderia contaminants, TSB4 was B. latens, SMB2 was B. cepacia, and SMB4 was Burkholderia sp. Phosphate solubilizing fungi SMJ3 isolate was Talaromyces muroii and SMJ6 was Talaromyces sp. The growth curve of the phosphate solubilizing bacteria showed a different pattern, likewise phosphate solubilizing fungi.

Keywords: Isolation; Identification; Phosphate Solubilizing Microbes; Sound