Influence of phenolic compounds and flavonoids on the colour and antioxidant activity of Melipona beecheii honey from deciduous forest of Yucatan, Mexico


  • Ina Ramírez Miranda Posgrado Institucional de Ciencias Agropecuarias y Manejo de Recursos Naturales y Tropicales, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
  • David Betancur Ancona Facultad de Ingeniería Química, Universidad Autónoma de Yucatán, Periférico Norte Km. 33.5, Tablaje Catastral 13615, Colonia Chuburná de Hidalgo Inn, 97203 Mérida, Yucatán, México.
  • Yolanda Moguel Ordóñez Campo Experimental Mocochá, CIRSE-INIFAP, Km 25 antigua carretera Mérida-Motul, Mocochá, Yucatán, México



The Melipona beecheii honey has bioactive compounds with high antioxidant activity that influence its colour. This research evaluated the influence of phenolic compounds and flavonoids on the colour and antioxidant activity of M. beecheii honeys. Forty-three honey samples from different site located in the low deciduous forest of Yucatan, Mexico, were extracted during the harvest and post-harvest seasons (2020 – 2021). The Pfund method was used to measure colour, Folin-Ciocalteu method for phenols measurement, aluminum chloride method for flavonoids determination, and ABTS and DPPH IC50 assay to determine antioxidants. The predominant colour of the honey was extra light amber and light amber at both seasons. Phenols ranged from 780 to 1317 mg/kg GAE; flavonoids from 36 to 55 mg/kg CE and antioxidant were 675 to 1161 µmol/kg TEAC by the ABTS and 361.8 to 173.0 mg/mL by the DPPH IC50.  Phenols and flavonoids were correlated with colour and radical scavenging activity, indicating the influence of these compounds with the darker colour and higher antioxidant activity of honey. The statistically significant difference (P<0.05) between seasons could be associated with the botanical origin with great diversity of melliferous flora present in the low deciduous forest of the Yucatan Peninsula.


Keywords: Antioxidant; colour; flavonoids; honey; Melipona beecheii.


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How to Cite

Miranda, I. R., D. B. Ancona, and Y. M. Ordóñez. “Influence of Phenolic Compounds and Flavonoids on the Colour and Antioxidant Activity of Melipona Beecheii Honey from Deciduous Forest of Yucatan, Mexico”. Emirates Journal of Food and Agriculture, vol. 35, no. 12, Dec. 2023, doi:10.9755/ejfa.2023.3198.



Research Article