DIFFERENCE ON ITS REGIONS AMONG YACON GENOTYPES AND SMALLANTHUS SPP.

Jana Ziarovska; Eloy Fernandez Cusimamani; Daniela Russo; Luigi Milella

doi:10.9755/ejfa.v26i1.16667

Authors

Jana Ziarovska FAPZ, Slovak Agricultural University, Nitra, Slovakia Faculty of Tropical Agrisciences, CULS ? Prague, Czech Republic Department of Science, University if Basilicata, Potenza, Italy
Eloy Fernandez Cusimamani FAPZ, Slovak Agricultural University, Nitra, Slovakia Faculty of Tropical Agrisciences, CULS ? Prague, Czech Republic Department of Science, University if Basilicata, Potenza, Italy
Daniela Russo FAPZ, Slovak Agricultural University, Nitra, Slovakia Faculty of Tropical Agrisciences, CULS ? Prague, Czech Republic Department of Science, University if Basilicata, Potenza, Italy
Luigi Milella FAPZ, Slovak Agricultural University, Nitra, Slovakia Faculty of Tropical Agrisciences, CULS ? Prague, Czech Republic Department of Science, University if Basilicata, Potenza, Italy

DOI:

https://doi.org/10.9755/ejfa.v26i1.16667

Keywords:

ITS region, Landraces, Molecular markers, DNA sequence, Yacon

Abstract

Yacon (Smallanthus sonchifolius) is an Andean crop which is very regarded for its medicinal properties. Yacon genetic profiles have been slightly studied using molecular markers. The aim of this study was to prepare the protocol for direct sequencing of the ITS (Internal Transcribed Spacers) regions of yacon DNA genome. The identified ITS regions in three Bolivian yacon's landraces (BOL 20, BOL 22 and BOL 24) were sequenced and then compared with ITS regions found in NCBI database, previously isolated in yacon and in other plants belonging to the genus Smallanthus. The analysed ITS regions of studied Bolivian landraces showed differences in the nucleotides 163-164 and 235-236 with the sequence previously isolated in yacon. A dendrogram was constructed by comparing the sequence presented in the manuscript and those in the NCBI database for Smallanthus, spp. In the dendrogram, the previously reported ITS sequence of Smallanthus sonchifolius was also used. Specific sites of Smallanthus sonchifolius ITS region gives a promising base for molecular approach identification of this underutilized crop.