EXTRACTION OF MANGIFERIN FROM MANGIFERA INDICA L. LEAVES USING MICROWAVE-ASSISTED TECHNIQUE

Suslebys Salomon; Iliana Sevilla; Rafael Betancourt; Aylema Romero; Lauro Nuevas-Paz; Jhoany Acosta-Esquijarosa

doi:10.9755/ejfa.v26i7.18188

Authors

Suslebys Salomon Centro de Investigación y Desarrollo de Medicamentos (CIDEM), 26 y 51, Plaza, La Habana, Cuba
Iliana Sevilla Centro de Ingeniería de Procesos (CIPRO), Facultad de Ingeniería Química, Instituto Superior Politécnico José A. Echeverría, Calle 114 No. 11901 e/ 119 y 127, Marianao, La Habana, Cuba
Rafael Betancourt Centro de Ingeniería de Procesos (CIPRO), Facultad de Ingeniería Química, Instituto Superior Politécnico José A. Echeverría, Calle 114 No. 11901 e/ 119 y 127, Marianao, La Habana, Cuba
Aylema Romero Centro de Investigación y Desarrollo de Medicamentos (CIDEM), 26 y 51, Plaza, La Habana, Cuba
Lauro Nuevas-Paz Centro Nacional de Genética Médica, Ave, 31 y 146, Rpto Cubanacán, Playa, La Habana, Cuba
Jhoany Acosta-Esquijarosa Centro de Ingeniería de Procesos (CIPRO), Facultad de Ingeniería Química, Instituto Superior Politécnico José A. Echeverría, Calle 114 No. 11901 e/ 119 y 127, Marianao, La Habana, Cuba

DOI:

https://doi.org/10.9755/ejfa.v26i7.18188

Keywords:

Mangiferin, Mangifera indica, Microwave assisted extraction (MAE), HPLC

Abstract

Mangiferin, a bioactive metabolite having potent antioxidant and pharmacological properties has been obtained in this work by microwave assisted extraction (MAE) technique, from Mangifera indica leaves. A Box–Behnken design was used to evaluate the effect of three parameters: microwave power, the time of extraction, and the ratio of solvent to raw material (mL/g) over the mangiferin yield. Irradiation time was the most important parameter on the recovery of mangiferin. The results indicated that the optimal conditions should be as follows: power of microwave 900 W, extraction time of 5 min and a solvent volume / vegetal material relation of 10. With these parameters the maximum mangiferin yield was 63.22%. The MAE process was compared with the conventional extraction in stirred tank. No significant statistical differences were found (p= 0.5639) between both extraction methods of mangiferin. The presence of the metabolite was conﬁrmed using high performance liquid chromatography. The method was validated with the guidelines set on the International Conference on Harmonization (ICH), for the validation of analytical procedures.