TAXONOMY, PHYLOGENY, CULTIVATION AND BIOLOGICAL ACTIVITIES OF A LENTINUS SPECIES FROM ANDMAN & NICOBAR ISLANDS (INDIA)

Ved Parkash Sharma; Shwet Kamal; Ramesh Chandra Upadhyay; Satish Kumar; Sanjeev Kumar Sanyal; Manjit Singh

doi:10.9755/ejfa.2015.04.135

Authors

Ved Parkash Sharma Directorate of Mushroom Research (Indian Council of Agricultural Research)Chambaghat, Solan (HP) –
Shwet Kamal ICAR-Directorate of Mushroom Research, Chambaghat, Solan, (HP) – 173213, India
Ramesh Chandra Upadhyay ICAR-Directorate of Mushroom Research, Chambaghat, Solan, (HP) – 173213, India
Satish Kumar ICAR-Directorate of Mushroom Research, Chambaghat, Solan, (HP) – 173213, India
Sanjeev Kumar Sanyal ICAR-Directorate of Mushroom Research, Chambaghat, Solan, (HP) – 173213, India
Manjit Singh ICAR-Directorate of Mushroom Research, Chambaghat, Solan, (HP) – 173213, India

DOI:

https://doi.org/10.9755/ejfa.2015.04.135

Keywords:

Mushroom, Lentinus sajor-caju, Taxonomy, Phylogeny, Cultivation, Nutrition

Abstract

Fruit bodies of Lentinus were collected from subtropical climate of Andman & Nicobar Islands (India). Pure culture was prepared by tissue culture on Malt-extract-agar (MEA) medium at 25C±2C. The specimen was studied for its identification, taxonomy and phylogeny and identified as Lentinus sajor-caju by morphological and microscopical studies. The identification was confirmed through ITS 5.8S rDNA sequencing and sequence analysis. Its cultivation was done on saw dust and wheat bran. The fruit bodies were obtained at a temperature of 28±2°C and 80-85% RH. The cultivated Lentinus sajor-caju fruit bodies were analysed for nutritional and biological properties and observed that the cultivated mushroom has good nutritional properties while antioxidant, reducing and DPPH free radical scavenging properties are comparable to commercially cultivated Lentinula edodes strains.