QUALITATIVE ANALYSIS OF AN ETHANOLIC EXTRACT FROM TRAMETES VERSICOLOR AND BIOLOGICAL SCREENING AGAINST LEISHMANIA AMAZONENSIS.
Keywords:Trametes versicolor, Leishmania amazonensis, metabolites
This study was carried out with basidiomycete mushroom Trametes versicolor. The fruiting body of thementioned mushroom was extracted with alcohol and submitted to a qualitative phytochemical screening for secondary metabolites identification. This experiment afforded the presence of secondary metabolites kind of triterpenes, steroids, flavonoids, alkaloids, and in a small amount cardenolides. Quinones were not found within tested metabolites. In addition, extracts of this mushroom fresh fruiting body of different polarity (ranging from n-hexane, dichloromethane, ethyl acetate until ethanol) were evaluated against promastigotes of Leishmania amazonensis, a widespread tropical and subtropical disease produced by around 20 species of protozoa from Leishmania genus. The ethyl acetate and the ethanol extract exhibited anti-leishmanial activity, with IC50 values of 101.8 ± 4.2 µg/mL and 97.4 ± 2.0 µg/mL, respectively. The cytotoxicity against peritoneal macrophage from BALB/c mice from those extracts was reported.