QUALITATIVE ANALYSIS OF AN ETHANOLIC EXTRACT FROM TRAMETES VERSICOLOR AND BIOLOGICAL SCREENING AGAINST LEISHMANIA AMAZONENSIS.

Vivian Leliebre-Lara; Marley Garcia; Clara Nogueiras; Lianet Monzote

doi:10.9755/ejfa.2015.05.194

Authors

Vivian Leliebre-Lara Centre for Natural Product Studies, Faculty of Chemistry, University of Havana, Cuba;
Marley Garcia Parasitology Department, Institute of Tropical Medicine “Pedro Kouri”, Havana, Cuba
Clara Nogueiras Centre for Natural Product Studies, Faculty of Chemistry, University of Havana, Cuba;
Lianet Monzote Parasitology Department, Institute of Tropical Medicine “Pedro Kouri”, Havana, Cuba

DOI:

https://doi.org/10.9755/ejfa.2015.05.194

Keywords:

Trametes versicolor, Leishmania amazonensis, metabolites

Abstract

This study was carried out with basidiomycete mushroom Trametes versicolor. The fruiting body of thementioned mushroom was extracted with alcohol and submitted to a qualitative phytochemical screening for secondary metabolites identification. This experiment afforded the presence of secondary metabolites kind of triterpenes, steroids, flavonoids, alkaloids, and in a small amount cardenolides. Quinones were not found within tested metabolites. In addition, extracts of this mushroom fresh fruiting body of different polarity (ranging from n-hexane, dichloromethane, ethyl acetate until ethanol) were evaluated against promastigotes of Leishmania amazonensis, a widespread tropical and subtropical disease produced by around 20 species of protozoa from Leishmania genus. The ethyl acetate and the ethanol extract exhibited anti-leishmanial activity, with IC50 values of 101.8 ± 4.2 µg/mL and 97.4 ± 2.0 µg/mL, respectively. The cytotoxicity against peritoneal macrophage from BALB/c mice from those extracts was reported.