@article{Nalla_Sharma_Sharma_2021, title={Mapping and validation of markers linked to Rf gene in sweet pepper (Capsicum annuum var grossum)}, volume={33}, url={https://ejfa.me/index.php/journal/article/view/2717}, DOI={10.9755/ejfa.2021.v33.i6.2717}, abstractNote={<p>Cytoplasmic male sterility (CMS), is the most valuable system in commercial hybrid seed production in hot pepper. Whereas in sweet pepper (<em>Capsicum annuum </em>var grossum), the unavailability of a stable restorer’s line is one of the major constraints in commercial hybrid breeding. Identifying the markers linked to CMS and nuclear restorer-of-fertility (<em>Rf</em>) genes will help in the production of CMS hybrids in sweet pepper. In the present study, we identified seven markers co-segregating with <em>Rf </em>genes using bulk segregating analysis (BSA) on the F<sub>2</sub> <em>Rf</em>-segregating mapping population. Among these, the CRF-SCAR marker proved to be linked to the fertility restorer gene and it could be used for screening of genotypes to identify restorers and non-restorer lines in future <em>Capsicum</em> breeding programs and help in the establishment of CMS system for commercial hybrid seed production.</p>}, number={6}, journal={Emirates Journal of Food and Agriculture}, author={Nalla, Manoj Kumar and Sharma, Vinod Kumar and Sharma, Vinukonda Rakesh}, year={2021}, month={Aug.}, pages={443–449} }