@article{Mansour_El-Ramah_C. Silveira_Bernardes_2022, title={An easy, safe, and practical method for semen collection in dromedary camels}, volume={34}, url={https://ejfa.me/index.php/journal/article/view/2850}, DOI={10.9755/ejfa.2022.v34.i4.2850}, abstractNote={<p>In the current study, we have developed an easy, practical, and reliable method to collect semen from camel bulls. Simply, intromission of<br>a super-sensitive artificial insemination glove intravaginally with an outer fixation in the perineal region from lateral and dorsal sides of the<br>vulva, mating, glove withdrawal and semen collection were the successive steps. Bull behaviour before and during the mating process was<br>observed. The collected semen was evaluated in aspects of semen colour, volume and sperm cell concentration. Also, individual sperm<br>motility and semen fertility by applying in-vitro fertilization were investigated after semen dilution in a new developed extender for camel<br>semen (100 mmol/l Tris, 65 mmol/l lactose, 50 mmol/l glucose, 40 mmol/l galactose, 125 mg/100ml BSA, 50 IU/ml catalase enzyme,<br>1000 IU/ml penicillin G and 500µg/ml streptomycin: pH 7.0). Presence of the intravaginal glove and its fixation by the external adhesive<br>tape did not affect the bull willingness and did not interrupt the mating process. Semen collection was successful in 112 out of total<br>128 trials (87.5%) and the lengthy copulation was (10.3 ± 6.2 min - Mean ± SD). Mean parameters of the collected ejaculates were:<br>volume of 8.3 ± 3.6 ml (Mean ± SD); sperm cell concentration of 437.6 ± 62.4 X 106 (Mean ± SD) sperm/ml; and sperm individual<br>motility after liquefaction for 45 min was 68.2 ± 11.4 % (Mean ± SD). Semen was grayish white to creamy white in colour depending<br>on sperm cell concentration. Finally, using of the collected diluted semen in IVF technique showed cleaved, morula and blastocyst rates<br>66.1%, 43.5% and 25.8%, respectively. In conclusion, using of intravaginal super-sensitive AI gloves is a practical technique for semen<br>collection and will facilitate the application of the new reproductive technologies on a large scale in dromedary camels.<br>Keywords: Semen collection, Camel, In-vitro fertilization</p>}, number={4}, journal={Emirates Journal of Food and Agriculture}, author={Mansour, Nabil and El-Ramah, Ahmed and C. Silveira, Marcia and Bernardes, Lucia A.M.}, year={2022}, month={May} }