TY - JOUR AU - Eldeen, Amira M. Gamal- AU - Raafat, Bassem M. AU - Daly, Sherien M. ‎El- AU - Fahmy, Cinderella A. AU - Almehmadi, Mazen M AU - Althobaiti, Fayez PY - 2021/08/18 Y2 - 2024/03/29 TI - Inhibitory effect of Sargassum latifolium extract on hypoxia pathway in colon cancer cells JF - Emirates Journal of Food and Agriculture JA - Emir J Food Agric VL - 33 IS - 7 SE - Research Article DO - 10.9755/ejfa.2021.v33.i7.2727 UR - https://ejfa.me/index.php/journal/article/view/2727 SP - 600-606 AB - <p><em>Sargassum latifolium, </em>‎(Turner) C. Agarth, 1820, <strong><em>‎</em></strong>is an edible brown alga that was collected from red seashores in Egypt. Colon cancer is a lethal disease world-wide. Hypoxia is a key player in progressive colon tumor growth and stemness. This work was planned to extract water-soluble polysaccharide from <em>S. </em><em>latifolium</em>, to separate its fractions (SL1, SL2, SL3, and SL4) and hence to investigate their anti-hypoxia characteristics in colon cancer HCT-116 cells. Algal fractions cytotoxicity was assayed by MTT; DNA staining was used to analyze apoptosis and necrosis; total hypoxia status was assessed by pimonidazole, HIF-1α and HIF-1β were estimated by ELISA, and hsa-miRNA-21-5p and hsa-miRNA-210-3p were analyzed by qPCR. The results indicated that SL1 and SL4 are cytotoxic agents against HCT-116 cells through enhancing apoptosis. SL1and SL4 were potent inhibitor of total cell hypoxia (<em>p</em> &lt; 0.001). Both fractions significantly suppressed the expression of miR-21 (<em>p</em> &lt; 0.01) and miR-210 (<em>p</em> &lt; 0.001), and the concentration of HIF-1α protein (<em>p</em> &lt; 0.01 and<em> p</em> &lt; 0.001, respectively), while only SL1 inhibited HIF-1β protein concentration (<em>p</em> &lt; 0.05). Taken together <em>S. latifolium</em> polysaccharide extract fractions SL1 and SL4 exhibited anti-hypoxic property in HCT-116 cells through mechanistic role in the expression of hypoxia regulators miRNA-21 and miRNA-210, and accordingly in HIF-1α and HIF-1β biosynthesis‎.</p> ER -