TY - JOUR AU - Rocha Barbosa, Iandra AU - Costa Nobre, Danúbia Aparecida AU - Ferreira de Oliveira, Denilson AU - Silva, Geraldo AU - Macedo, Willian Rodrigues PY - 2022/06/27 Y2 - 2024/03/29 TI - New insights into the mode of action of thiamethoxam on seed germination physiology JF - Emirates Journal of Food and Agriculture JA - Emir J Food Agric VL - 34 IS - 5 SE - Research Article DO - 10.9755/ejfa.2022.v34.i5.2857 UR - https://ejfa.me/index.php/journal/article/view/2857 SP - AB - <p>In order to contribute to understanding the mode of action of the thiamethoxam in plants, we have carried out a virtual screening and <br>in silico molecular docking study with TIA. The pharmacophoric search for protein ligands with three-dimensional structures similar to <br>TIA resulted in several protein ligands with Tanimoto score equal to or greater tha 0.5, but only the ligand isoniazid (ISZ) was bound to <br>a protein from plant origin, which was an ascorbate peroxidase (APX). Molecular docking of ISZ and TIA to several APX’s revealed that <br>TIA binds more strongly to these enzymes than ISZ, suggesting that TIA acts on plants through inhibition of this enzyme. When seedlings <br>obtained from wheat and soybean seeds treated with different concentrations ot TIA, the activity of APX decreased with increasing TIA <br>concentration. Therefore, both in silico and experimental results suggest that TIA can promotes plant growth through inhibition of the <br>enzyme APX</p> ER -