GENETIC RELATIONSHIP BETWEEN ROSELLE (HIBISCUS SABDARIFFA L.) AND KENAF (HIBISCUS CANNABINUS L.) ACCESSIONS THROUGH OPTIMIZATION OF PCR BASED RAPD METHOD

Authors

  • Omalsaad Mohammed School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia
  • A. K. M. Aminul Islam Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh
  • Murshida Akhter Jahan B-1/8, BSMRAU Campus, Gazipur 1706, Bangladesh
  • Zahira Yaakob Department of Chemical and Process Engineering, Faculty of Engineering & Built Environment, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia
  • Mohamad Osman School of Environmental and NaturaFaculty of Plantation and Agrotechnology, Universiti Technology Mara (UiTM), Shah Alam, Malaysial Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia Department of Genetics and Plant Breeding, Faculty of Agriculture, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur 1706, Bangladesh B-1/8, BSMRAU Campus, Gazipur 1706, Bangladesh Department of Chemical and Process Engineering, Faculty of Engineering & Built Environment, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor 43600, Malaysia 5 Faculty of Plantation and Agrotechnology, Universiti Technology Mara (UiTM), Shah Alam, Malaysia

DOI:

https://doi.org/10.9755/ejfa.v26i3.16498

Keywords:

DNA extraction, RAPD-PCR optimization, genetic similarity, phylogenetics

Abstract

The PCR based RAPD analysis was used to study genetic relationship among roselle and kenaf accessions. Extraction of genomic DNA samples of nine roselle (Hibiscus sabdariffa L.) and seven kenaf (Hibiscus cannabinus L.) accessions were successfully performed by Cetyl Trimethyl Ammonium Bromide (CTAB) method. RAPD analysis using two chloroplast primers and one mitochondrial primer produced about 62 bands of different sizes. Most of the fragments were polymorphic where 2-3 fragments were monomorphic in each primer. Sixteen accessions were classified into two main groups A and B through dendogram generated using RAPD polymorphism. Five kenaf accessions were included in group A, whereas nine roselle accessions and two kenaf accessions were included in group B. A group was formed with a range of similarity coefficients of 0.5333 to 0.8780 having all roselle accessions. Bengkalis was different from Hibiscus sp but it was close to roselle accessions. Noonsoon was very close to roselle accessions having a range of jaccard similarity coefficients of 0.5000 to 0.6326. It was evident from jaccad similarity coefficient of 0.8780 that the roselle varieties UKMR 1 and UKMR 2 were genetically closer to each other. On the other hand, V36 and Thainung showed the highest similarity coefficient (0.7878) among kenaf accessions in group A. This study has successfully differentiated between two species by using chloroplast and mitochondrial markers which was due to high level of polymorphism. However, the chloroplast primers were more effective than M13 to study the genetic variation between roselle and kenaf.

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Published

2013-10-11

How to Cite

Mohammed, O., A. K. M. A. Islam, M. A. Jahan, Z. Yaakob, and M. Osman. “GENETIC RELATIONSHIP BETWEEN ROSELLE (HIBISCUS SABDARIFFA L.) AND KENAF (HIBISCUS CANNABINUS L.) ACCESSIONS THROUGH OPTIMIZATION OF PCR BASED RAPD METHOD”. Emirates Journal of Food and Agriculture, vol. 26, no. 3, Oct. 2013, pp. 247-58, doi:10.9755/ejfa.v26i3.16498.

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